Occurrence of 7-methylguanine in nucleic acids of rat liver.

نویسندگان

  • V M Craddock
  • S Villa-Trevino
  • P N Magee
چکیده

1. Microsomal and soluble RNA of rat liver have been studied by column and paper chromatography after administration of [Me-(14)C]methionine; evidence was obtained for the occurrence of 7-methylguanine, the methyl group being derived from methionine. 2. No evidence was obtained for the occurrence of 7-methylguanine in DNA.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Interaction of the carcinogen 3,3-dimethyl-1-phenyltriazene with nucleic acids of various rat tissues and the effect of a protein-free diet.

The methylation by 3,3-[14C]dimethyl-1-phenyltriazene of nucleic acids in various rat tissues was investigated. Following a single s.c. injection of 3,3-[14C]dimethyl-1-phenyltriazene (50 mg/kg), approximately 40% of the radioactivity was subsequently exhaled as 14CO2. Expiration of 14CO2, metabolic 14C labeling of liver proteins, and formation of 7-[14C]methylguanine, the major reaction produc...

متن کامل

Interaction of the Carcinogen 3,3-Dimethyl- l -phenyltr iazene with Nucleic Acids of Various Rat Tissues and the Effect of a Protein-free Diet 1

The methylation by 3,3-[~4C]dimethyl-l-phenyltriazene of nucleic acids in various rat tissues was investigated. Following a single s.c. injection of 3,3-['4C]dimethyl-l-phenyl triazene (50 mg/kg), approximately 40% of the radioactivity was subsequently exhaled as ~4CO2. Expiration of '4CO2, metabolic ]4C labeling of liver proteins, and formation of 7[~4C]methylguanine, the major reaction produc...

متن کامل

The Alkylation of Nucleic Acids of the Rat by N-methyl-n-nitrosourea, Dimethylnitrosamine, Dimethyl Sulphate and Methyl Methanesulphonate

1. N[14C]-Methyl-N-nitrosourea, [14C]dimethylnitrosamine, [14C]dimethyl sulphate and [14C]methyl methanesulphonate were injected into rats, and nucleic acids were isolated from several organs after various time-intervals. Radioactivity was detected in DNA and RNA, partly in major base components and partly as the methylated base, 7-methylguanine. 2. No 7-methylguanine was detected in liver DNA ...

متن کامل

Occurrence of Methylated Purine Bases in Yeast Ribonucleic Acid*

A number of methylated purine bases have recently been reported to occur in small quantities in the urine of normal human subjects. These include 1-methylguanine, iV*-methylguanine (6-hydroxy-2-methylaminopurine) ,I 1-methylhypoxanthine, and 8-hydroxy-7-methylguanine (1) in addition to 7-methylguanine, previously known as a urinary constituent (2). These compounds were found to be metabolites o...

متن کامل

Stability of deoxyribonucleic acid methylated in the intact animal by administration of dimethylnitrosamine. Rate of breakdown in vivo and in vitro at different dosages.

1. The effect of administration of various dosages of dimethylnitrosamine on the extent of methylation of liver and kidney nucleic acids in the intact rat was studied. Methylation of liver nucleic acids was linearly related to the dosage, but decreasing the dose produced relatively less lowering of the extent of alkylation of kidney nucleic acids. 2. The rates of disappearance of 7-methylguanin...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • The Biochemical journal

دوره 107 2  شماره 

صفحات  -

تاریخ انتشار 1968